We used qRT-PCR and DNA microarrays to characterize the transcriptional profile of human MS brain lesions (Baranzini et al. J Immunol; 2000) and mouse CNS and peripheral organs after induction of inflammatory demyelination (EAE) (Baranzini et al. J Immunol; 2005; Otaegui et al. J Immunol 2007). We are currently using massively parallel sequencing to investigate patterns of gene expression in human white matter and grey matter post-mortem tissue.
Methods: Molecular biology, advanced statistics, bioinformatics